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SH-4-54

(CAS No:1456632-40-8)

SH-4-54 is a potent STAT inhibitor with KD of 300 nM and 464 nM for STAT3 and STAT5, respectively.

CAS No:1456632-40-8

Molecular Weight(MW):610.59

Purity:98%+

Specification:500mg;1g;5g;10g;50g;100g

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QC Documents

COA	MSDS	HPLC	NMR

ChemicalInfomation

CAS No:	1456632-40-8
Molecular formula(MF)	C₂₉H₂₇F₅N₂O₅S
Molecular Weight(MW):	610.59
Alias

Solubility

In vitro	DMSO	100 mg/mL (163.77 mM)
	Ethanol	50 mg/mL warmed (81.88 mM)
	Water	<1 mg/mL
In vivo

Biological Activity

Description

SH-4-54 is a potent STAT inhibitor with K_D of 300 nM and 464 nM for STAT3 and STAT5, respectively.

Targets

STAT3 ^[1]	STAT5 ^[1]
300 nM(Kd)	464 nM(Kd)

In vitro

SH-4-54 shows unprecedented cytotoxicity in human glioblastoma brain cancer stem cells (BTSCs), while has no toxicity in human fetal astrocytes. In addition, SH-4-54 effectively suppresses STAT3 phosphorylation and its downstream transcriptional targets. ^[1]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
human 127EF cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 127EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=66 nM	24900612
human 30M cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 30M cells assessed as cell viability after 3 days by Alamar Blue assay, EC50=0.1 Î¼M	24900612
human 84EF cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 84EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.102 Î¼M	24900612
human 67EF cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 67EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.106 Î¼M	24900612
human 73EF cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 73EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.162 Î¼M	24900612
human 25EF cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 25EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.234 Î¼M	24900612
human 73M cells	CytotoxicÂ assay	3 days	Cytotoxicity against human 73M cells assessed as cell viability after 8 days by Alamar Blue assay, IC50=1.03 Î¼M	24900612
human 147EF cells	Function assay	2-72 h	Inhibition of STAT3 in human 147EF cells assessed as reduction of phosphorylated cyclin D1 level after 2 to 72 hrs by Western blotting analysis	24900612

... Click to View More Cell Line Experimental Data

In vivo

In mice orthotopically xenografted with BT73, SH-4-54 (10 mg/kg i.p.) exhibits BBB permeability, potently suppresses glioma tumor growth, and inhibits pSTAT3. ^[1]